: The protein is expressed in a culture medium at 37∘C37 raised to the composed with power C
Depending on the field, "preparing a piece" for a 991 might mean: RBD-991
: The target fragment (bases 991–1749) corresponding to amino acids 331–583 is synthesized to represent the Receptor-Binding Domain (RBD). Vector Digestion : An expression vector, such as pQE30 : The protein is expressed in a culture